B-Raf Antibody (BRAF-V600E/13276R) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-49527

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-49527
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # BRAF-V600E/13276R

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all B-Raf Antibodies »

Product Specifications

Immunogen

Recombinant fragment corresponding to human BRAF V600E mutant protein (Uniprot: P15056)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml. Also available with 0.05% BSA & 0.05% azide.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Scientific Data Images for B-Raf Antibody (BRAF-V600E/13276R) - Azide and BSA Free

B-Raf Antibody (BRAF-V600E/13276R) - Azide and BSA Free

Immunohistochemistry-Paraffin: B-Raf Antibody (BRAF-V600E/13276R) - Azide and BSA Free [NBP3-49527] -

Formalin-fixed, paraffin-embedded human melanoma stained with B-Raf Antibody (BRAF-V600E/13276R) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.

Applications for B-Raf Antibody (BRAF-V600E/13276R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

Additional B-Raf Products

Product Documents for B-Raf Antibody (BRAF-V600E/13276R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for B-Raf Antibody (BRAF-V600E/13276R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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