ATG4B Antibody (23GB3240)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51209

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NBP3-51209-100ul
NBP3-51209-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB3240

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATG4B Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human ATG4B (Uniprot: Q9Y4P1)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATG4B Antibody (23GB3240)

ATG4B Antibody (23GB3240)

Immunocytochemistry/ Immunofluorescence: ATG4B Antibody (23GB3240)[NBP3-51209] -

Immunocytochemical staining of C2C12 cells with ATG4B (1:1,000). Nuclei were stained blue with DAPI; ATG4B was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
ATG4B Antibody (23GB3240)

Flow Cytometry: ATG4B Antibody (23GB3240)[NBP3-51209] -

Validation of ATG4B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ATG4B antibody (1:2,000) and analyzed using BD flow cytometer.
ATG4B Antibody (23GB3240)

Flow Cytometry: ATG4B Antibody (23GB3240)[NBP3-51209] -

Flow cytometric analysis of ATG4Bexpression in C2C12 cells using ATG4B antibody (1:2,000). Green, isotype control; red, ATG4B.

Applications for ATG4B Antibody (23GB3240)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATG4B

FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 5 named isoforms produced by alternative splicing.

Long Name

ATG4 Autophagy Related 4 Homolog B

Alternate Names

Apg4B, AUTL1

Gene Symbol

ATG4B

Additional ATG4B Products

Product Documents for ATG4B Antibody (23GB3240)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATG4B Antibody (23GB3240)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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