ATG12 Antibody (948) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43752

Novus Biologicals, part of Bio-Techne
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NBP2-43752
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 948

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATG12 Antibodies »

Product Specifications

Immunogen

Recombinant protein corresponding to human ATG12

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Novus Biologicals Mouse ATG12 Antibody (948) - Azide and BSA Free (NBP2-43752) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ATG12 Antibody (948) - Azide and BSA Free

Western Blot: ATG12 Antibody (948) [NBP2-43752]

Western Blot: ATG12 Antibody (948) [NBP2-43752]

Western Blot: ATG12 Antibody (948) [NBP2-43752] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody [GT948].
Immunocytochemistry/ Immunofluorescence: ATG12 Antibody (948) [NBP2-43752]

Immunocytochemistry/ Immunofluorescence: ATG12 Antibody (948) [NBP2-43752]

Immunocytochemistry/Immunofluorescence: ATG12 Antibody (948) [NBP2-43752] - ATG12 Antibody (948) detects ATG12 protein at autophagosome by immunofluorescent analysis. Sample: Mock and treated HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: ATG12 stained by ATG12 Antibody (948) diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: ATG12 Antibody (948) [NBP2-43752]

Western Blot: ATG12 Antibody (948) [NBP2-43752]

Western Blot: ATG12 Antibody (948) [NBP2-43752] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [948] dilution: 1:1000
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Applications for ATG12 Antibody (948) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATG12

The ATG12 gene codes a ubiquitin-like protein ATG12 that in isoform 1 is 140 amino acids long at 15 kDA and in isoform 2 is 74 amino acids long at 7 kDA. This protein is critical for autophagy (bulk protein degradation) as it is the human homolog of a yeast protein that participates in autophagy. ATG12 participates in the immune system, apoptosis and autophagy, protein stability, negative regulators of RIG-I/MDA5 signaling, and glucose/energy metabolism. It is known to interact with various genes such as HIST1H2BF, HIST1H2BE, HIST1H2BI, HIST1H2BG, and HIST1H2BC. ATG12 has been researched regarding its role in leukemia, lung cancer, myositis, retinitis, carcinoma, colorectal cancer, and myelodysplastic syndrome.

Long Name

ATG12 Autophagy Related 12 Homolog

Alternate Names

APG12, FBR93, HAPG12

Gene Symbol

ATG12

Additional ATG12 Products

Product Documents for ATG12 Antibody (948) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATG12 Antibody (948) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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