ASPP1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90078

Novus Biologicals, part of Bio-Techne
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NBP1-90078
NBP1-90078-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ASPP1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PNIQKLLYQRFNTLAGGMEGTPFYQPSPSQDFMGTLADVDNGNTNANGNLEELPPAQPTAPLPAEPAPSSDANDNELPSPEPEELICPQTTHQTAEPAEDNNNNVATVPTTEQIPSPVAEAPSPGEEQVPPAPLPPASHPPATSTNK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit ASPP1 Antibody - BSA Free (NBP1-90078) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ASPP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ASPP1 Antibody [NBP1-90078]

Immunocytochemistry/ Immunofluorescence: ASPP1 Antibody [NBP1-90078]

Immunocytochemistry/Immunofluorescence: ASPP1 Antibody [NBP1-90078] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
ASPP1 Antibody - BSA Free Immunohistochemistry: ASPP1 Antibody - BSA Free [NBP1-90078]

Immunohistochemistry: ASPP1 Antibody - BSA Free [NBP1-90078]

Staining of human duodenum shows strong nuclear positivity in glandular cells.
ASPP1 Antibody - BSA Free Immunohistochemistry: ASPP1 Antibody - BSA Free [NBP1-90078]

Immunohistochemistry: ASPP1 Antibody - BSA Free [NBP1-90078]

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
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Applications for ASPP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASPP1

The ASPP family members ASPP1, ASPP2, act as potent activators of p53. A third member of the family, iASPP, has an inhibitory effect on apoptosis. The three members of ASPP family all bind p53 via their C-terminus, which contains a proline-rich region, four ankyrin repeats and an SH3 domain. Expression of ASPP1 and ASPP2 is frequently downregulated in human breast tumors expressing wild-type p53. ASPP1 may also play a role in vascular development.

Alternate Names

apoptosis-stimulating protein of p53, 1, ASPP1Protein phosphatase 1 regulatory subunit 13B, KIAA0771apoptosis-stimulating of p53 protein 1, p53BP2-like, p85, protein phosphatase 1, regulatory (inhibitor) subunit 13B

Gene Symbol

PPP1R13B

Additional ASPP1 Products

Product Documents for ASPP1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ASPP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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