ASC/TMS1 Antibody - BSA Free

Immunohistochemistry: ASC/TMS1 Antibody - BSA Free [NBP1-78978]

Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78978] - Analysis of ASC/TMS1 in mouse intestine using DAB with hematoxylin counterstain.

Immunocytochemistry/ Immunofluorescence: ASC/TMS1 Antibody - BSA Free [NBP1-78978]

Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78978] - Tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-78978] -

NLRP3 inflammasome and GSDMD are strongly expressed and activated in CNS microglia during EAE.A Immunoblot analysis of NLRP3, ASC, full-length (FL) and cleaved caspase-1, IL-1 beta, and GSDMD in the lumbosacral spinal cords of EAE-induced WT mice at peak disease or CFA-treated mice on day 18 after treatment (n = 10 per group). B Immunohistochemistry showing infiltration of NLRP3, ASC, caspase-1, IL-1 beta, and GSDMD-positive immune cells in the spinal cords of EAE-induced WT mice at peak disease or CFA-treated WT mice on day 18 after treatment. Scale bar: 50 um. n = 10 per group. C Immunofluorescent labeling of Iba-1 (green), NLRP3, ASC, caspase-1, or GSDMD (red), and DAPI (blue) demonstrates the expression and process of NLRP3 inflammasome and GSDMD-mediated pyroptosis in the microglia of WT EAE mice at peak disease or CFA-treated WT mice on day 18 after treatment (n = 10 per group). Scale bar: 20 um. Data are from three representative independent experiments and were analyzed by an unpaired t-test or the Mann–Whitney U test. Error bars show the mean +/- SEM. ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36765034), licensed under a CC-BY license. Not internally tested by Novus Biologicals.