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ASAH1 Antibody (1A7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # H00000427-M02

Novus Biologicals, part of Bio-Techne
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H00000427-M02

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 1A7

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ASAH1 (NP_808592, 25 a.a. ~ 124 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPMLKVIVNSLKNMINTFVPSGKVMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTD

Specificity

ASAH1 - N-acylsphingosine amidohydrolase (acid ceramidase) 1 (1A7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ASAH1 Antibody (1A7) - Azide and BSA Free

Western Blot: ASAH1 Antibody (1A7) [H00000427-M02]

Western Blot: ASAH1 Antibody (1A7) [H00000427-M02]

Western Blot: ASAH1 Antibody (1A7) [H00000427-M02] - Analysis of ASAH1 expression in transfected 293T cell line by ASAH1 monoclonal antibody (M02), clone 1A7.Lane 1: ASAH1 transfected lysate(45 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: ASAH1 Antibody (1A7) [H00000427-M02]

Immunohistochemistry-Paraffin: ASAH1 Antibody (1A7) [H00000427-M02]

Immunohistochemistry-Paraffin: ASAH1 Antibody (1A7) [H00000427-M02] - Analysis of monoclonal antibody to ASAH1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
ELISA: ASAH1 Antibody (1A7) [H00000427-M02]

ELISA: ASAH1 Antibody (1A7) [H00000427-M02]

ELISA: ASAH1 Antibody (1A7) [H00000427-M02] - Detection limit for recombinant GST tagged ASAH1 is approximately 1ng/ml as a capture antibody.

Applications for ASAH1 Antibody (1A7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for IHC-P.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ASAH1

This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Acid ceramidase

Alternate Names

AC, ACDase, Acid CDase, ASAH, EC 3.5.1.-, EC 3.5.1.23, HSD-33, HSD33

Entrez Gene IDs

427 (Human)

Gene Symbol

ASAH1

Additional ASAH1 Products

Product Documents for ASAH1 Antibody (1A7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ASAH1 Antibody (1A7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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