ARP10 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91682
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence, SDS-PAGE
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CSSCAWELVDFIKAHDHLNLGIFASRLYYHWCKPQQKGLRLLCGSQVPVEVMGFPEFADCWENFVDHEKPLSFNPYKMLEELDKNSRAIKRRLERIKQS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ARP10 Antibody - BSA Free
Western Blot: ARP10 Antibody [NBP1-91682]
ARP10-Antibody-Western-Blot-NBP1-91682-img0005.jpgWestern Blot: ARP10 Antibody [NBP1-91682]
Western Blot: ARP10 Antibody [NBP1-91682] - A) APOBEC3H (A3H) haplotype II SV183 and various mutants expressed in 293T cells. B) The Novus anti-ARP10 antibody detected two splice variants of A3H, SV183 and SV200 in MCF-7 cell lysates. Transient transfection, overnight incubation with primary. WB image submitted by a verified customer review.Western Blot: ARP10 Antibody [NBP1-91682] -
Western Blot: ARP10 Antibody [NBP1-91682] - Generation & validation of HIV-1 Vif separation-of-function molecular/viral probes.A) A schematic of the Vif protein encoded by each HIV-1 molecular clone showing amino acid differences responsible for the hyper- & hypo-Vif functionality relative to lab-Vif (HIV-1 IIIB/NL4-3) against stable A3H haplotype II. B) Immunoblots showing the expression levels of the indicated A3 proteins stably expressed in SupT11 cells. In this experiment untagged A3H is detected with the mouse monoclonal antibody P3A3-A10. C) HIV-1 spreading infection kinetics for the indicated viruses on A3-expressing SupT11 cells lines described in panel B. The hyper-, lab-, & hypo-Vif isolates spread with similar kinetics on cells expressing a control vector, A3D, A3F, or A3G, but showed clear phenotypic differences on cells expressing low, intermediate (int), & high levels of stable A3H haplotype II. Delta-Vif virus replication was evident in control vector expressing SupT11 cells, delayed in A3D expressing cells, & suppressed under all other conditions (some symbols eclipsed). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1004761), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for ARP10 Antibody - BSA Free
Application
Recommended Usage
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval method is recommended.
Reviewed Applications
Read 1 review rated 5 using NBP1-91682 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ARP10
Alternate Names
APOBEC-related protein 10, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3H, ARP-10, ARP10Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3H, DNA dC->dU-editing enzyme APOBEC-3H, EC 3.5.4.-
Entrez Gene IDs
164668 (Human)
Gene Symbol
APOBEC3H
UniProt
Additional ARP10 Products
Product Documents for ARP10 Antibody - BSA Free
Product Specific Notices for ARP10 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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