ARID5A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81037
![ARID5A Antibody - BSA Free Immunohistochemistry-Paraffin: ARID5A Antibody [NBP1-81037]](https://resources.bio-techne.com/images/products/antibody/nbp1-81037_rabbit-polyclonal-arid5a-antibody-immunohistochemistry-paraffin-1710202515223.jpg "Immunohistochemistry-Paraffin: ARID5A Antibody [NBP1-81037]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LVLPYVRHLKGEDDKPLPTSKPRKQYKMAKENRGDDGATERPKKAKEERRMDQMMPGKTKADAADPAPLPSQEPPRNSTEQQGLASGSSVSFVGASGCPEAYKRLLSSFYCKGTH
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit ARID5A Antibody - BSA Free (NBP1-81037) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ARID5A Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: ARID5A Antibody [NBP1-81037]](https://resources.bio-techne.com/images/products/ARID5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81037-img0004.jpg "Immunocytochemistry/ Immunofluorescence: ARID5A Antibody [NBP1-81037]")
Immunocytochemistry/ Immunofluorescence: ARID5A Antibody [NBP1-81037]
Immunocytochemistry/Immunofluorescence: ARID5A Antibody [NBP1-81037] - Staining of human cell line A-431 shows positivity in nucleus and nucleoli.![Western Blot: ARID5A Antibody [NBP1-81037]](https://resources.bio-techne.com/images/products/antibody/HPA023879-wb-6jp.jpg "Western Blot: ARID5A Antibody [NBP1-81037]")
Western Blot: ARID5A Antibody [NBP1-81037]
Analysis in human cell line HDLM-2.![ARID5A Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: ARID5A Antibody - BSA Free [NBP1-81037]](https://resources.bio-techne.com/images/products/nbp1-81037_rabbit-polyclonal-arid5a-antibody-25620259121923.jpg "Chromatin Immunoprecipitation-exo-Seq: ARID5A Antibody - BSA Free [NBP1-81037]")
Chromatin Immunoprecipitation-exo-Seq: ARID5A Antibody - BSA Free [NBP1-81037]
ChIP-Exo-Seq composite graph for Anti-ARID5A (NBP1-81037) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.![ARID5A Antibody - BSA Free Immunohistochemistry-Paraffin: ARID5A Antibody [NBP1-81037]](https://resources.bio-techne.com/images/products/antibody/nbp1-81037_rabbit-polyclonal-arid5a-antibody-immunohistochemistry-paraffin-1710202515335.jpg "Immunohistochemistry-Paraffin: ARID5A Antibody [NBP1-81037]")
![ARID5A Antibody - BSA Free Immunohistochemistry-Paraffin: ARID5A Antibody [NBP1-81037]](https://resources.bio-techne.com/images/products/antibody/nbp1-81037_rabbit-polyclonal-arid5a-antibody-immunohistochemistry-paraffin-1710202515510.jpg "Immunohistochemistry-Paraffin: ARID5A Antibody [NBP1-81037]")
![ARID5A Antibody - BSA Free Immunohistochemistry-Paraffin: ARID5A Antibody [NBP1-81037]](https://resources.bio-techne.com/images/products/antibody/nbp1-81037_rabbit-polyclonal-arid5a-antibody-immunohistochemistry-paraffin-1710202515628.jpg "Immunohistochemistry-Paraffin: ARID5A Antibody [NBP1-81037]")
Applications for ARID5A Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ARID5A
Alternate Names
ARID domain-containing protein 5A, AT rich interactive domain 5A (MRF1-like), AT-rich interactive domain-containing protein 5A, RFVG5814
Entrez Gene IDs
10865 (Human)
Gene Symbol
ARID5A
UniProt
Additional ARID5A Products
Product Documents for ARID5A Antibody - BSA Free
Product Specific Notices for ARID5A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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