ARID1A Antibody (CL3595) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44007

Novus Biologicals, part of Bio-Techne
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NBP3-44007
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BSA Free
NBP2-61623
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3595

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ARID1A Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O14497, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse ARID1A Antibody (CL3595) - Azide and BSA Free (NBP2-61623) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ARID1A Antibody (CL3595) - Azide and BSA Free

ARID1A Antibody (CL3595) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: ARID1A Antibody [NBP3-44007]

Immunocytochemistry/ Immunofluorescence: ARID1A Antibody [NBP3-44007]

Staining of A-431 cells using the Anti-ARID1A monoclonal antibody) .
ARID1A Antibody (CL3595) - Azide and BSA Free Western Blot: ARID1A Antibody [NBP3-44007]

Western Blot: ARID1A Antibody [NBP3-44007]

Lane 1: Marker [kDa] 250)
Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells)
ARID1A Antibody (CL3595) - Azide and BSA Free Immunohistochemistry-Paraffin: ARID1A Antibody [NBP3-44007]

Immunohistochemistry-Paraffin: ARID1A Antibody [NBP3-44007]

Staining of human breast cancer shows strong nuclear positivity in tumor cells.
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Applications for ARID1A Antibody (CL3595) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARID1A

ARID1A encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain ARID1A genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ARID domain-containing protein 1A, AT rich interactive domain 1A (SWI- like), AT rich interactive domain 1A (SWI-like), AT-rich interactive domain-containing protein 1A, B120SWI-like protein, BAF250A, BAF250SWI/SNF complex protein p270, BM029, brain protein 120, BRG1-associated factor 250, BRG1-associated factor 250a, C10rf4, C1orf4SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatinsubfamily F member 1, chromatin remodeling factor p250, hELD, hOSA1, matrix associated, actin dependent regulator of chromatin, Osa homolog 1, OSA1, OSA1 nuclear protein, P270, SMARCF1, subfamily f, member 1

Gene Symbol

ARID1A

Additional ARID1A Products

Product Documents for ARID1A Antibody (CL3595) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ARID1A Antibody (CL3595) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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