Aprataxin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-534
![Western Blot: Aprataxin Antibody [NB100-534]](https://resources.bio-techne.com/images/products/Aprataxin-Antibody-Western-Blot-NB100-534-img0008.jpg "Western Blot: Aprataxin Antibody [NB100-534]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human Aprataxin using the numbering given in SwissProt Entry Q7Z2E3 (GeneID 54840).
Specificity
NB100-534 is specific for human Aprataxin protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Aprataxin Antibody - BSA Free (NB100-534) is a polyclonal antibody validated for use in WB and IP. Anti-Aprataxin Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Aprataxin Antibody - BSA Free
Western Blot: Aprataxin Antibody [NB100-534]
Western Blot: Aprataxin Antibody [NB100-534] - Detection of human Aprataxin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aprataxin antibody NB100-534 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Western Blot: Aprataxin Antibody [NB100-534]](https://resources.bio-techne.com/images/products/Aprataxin-Antibody-Western-Blot-NB100-534-img0002.jpg "Western Blot: Aprataxin Antibody [NB100-534]")
Western Blot: Aprataxin Antibody [NB100-534]
Western Blot: Aprataxin Antibody [NB100-534] - Whole cell lysate (50 ug) from normal human lymphoblasts (C3ABR cells; WT) or lymphoblasts from an AOA patient (738RM cells; AOA). Antibody used at 0.1 and 0.04 ug/ml.![Western Blot: Aprataxin Antibody [NB100-534]](https://resources.bio-techne.com/images/products/Aprataxin-Antibody-Western-Blot-NB100-534-img0007.jpg "Western Blot: Aprataxin Antibody [NB100-534]")
Western Blot: Aprataxin Antibody [NB100-534]
Western Blot: Aprataxin Antibody [NB100-534] - Detection of Human Aprataxin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aprataxin antibody NB100-534 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for Aprataxin Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
1 - 4 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:10,000 - 1:25,000. Immunoprecipitation: 1 to 4 ug/mg lysate. Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Aprataxin
Alternate Names
AOA1EAOH, AOAMGC1072, aprataxin, ataxia 1, early onset with hypoalbuminemia, AXA1, EC 3.-, EOAHA, FHA-HIT, FLJ20157, Forkhead-associated domain histidine triad-like protein
Entrez Gene IDs
7332 (Human)
Gene Symbol
UBE2L3
UniProt
Additional Aprataxin Products
Product Documents for Aprataxin Antibody - BSA Free
Product Specific Notices for Aprataxin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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