APP Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15575
![Western Blot: APP Antibody [NBP2-15575]](https://resources.bio-techne.com/images/products/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0006.jpg "Western Blot: APP Antibody [NBP2-15575]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Predicted:
Bovine (96%), Canine (93%), Chicken (92%), Chimpanzee (100%), Porcine (95%), Rabbit (94%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human APP. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (83%).
Localization
Membrane; Membrane; clathrin-coated pit
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for APP Antibody - BSA Free
![Western Blot: APP Antibody [NBP2-15575]](https://resources.bio-techne.com/images/products/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0008.jpg "Western Blot: APP Antibody [NBP2-15575]")
Western Blot: APP Antibody [NBP2-15575]
Western Blot: APP Antibody [NBP2-15575] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.![Immunohistochemistry-Paraffin: APP Antibody [NBP2-15575]](https://resources.bio-techne.com/images/products/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP2-15575-img0003.jpg "Immunohistochemistry-Paraffin: APP Antibody [NBP2-15575]")
Immunohistochemistry-Paraffin: APP Antibody [NBP2-15575]
Immunohistochemistry-Paraffin: APP Antibody [NBP2-15575] - Rat brain. APP antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Western Blot: APP Antibody [NBP2-15575]](https://resources.bio-techne.com/images/products/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0004.jpg "Western Blot: APP Antibody [NBP2-15575]")
Western Blot: APP Antibody [NBP2-15575]
Western Blot: APP Antibody [NBP2-15575] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody diluted at 1:500.![Western Blot: APP Antibody [NBP2-15575]](https://resources.bio-techne.com/images/products/beta-Amyloid-Antibody-Western-Blot-NBP2-15575-img0007.jpg "Western Blot: APP Antibody [NBP2-15575]")
Applications for APP Antibody - BSA Free
Application
Recommended Usage
ELISA
1:1000-1:10000
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Reviewed Applications
Read 1 review rated 4 using NBP2-15575 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: APP
Long Name
Amyloid Precursor Protein
Alternate Names
Amyloid beta, beta Amyloid, Protease Nexin II
Gene Symbol
APP
UniProt
Additional APP Products
Product Documents for APP Antibody - BSA Free
Product Specific Notices for APP Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.