AP1GBP1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36531
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by the antibody maps to a region between residue 975 to 1025 of human Gamma-synergin using the numbering given in entry NP_009178.3.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit AP1GBP1 Antibody - BSA Free (NBP2-36531) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for AP1GBP1 Antibody - BSA Free
Western Blot: AP1GBP1 Antibody [NBP2-36531]
Western Blot: AP1GBP1 Antibody [NBP2-36531] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Gamma-synergin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: AP1GBP1 Antibody [NBP2-36531]
Immunoprecipitation: AP1GBP1 Antibody [NBP2-36531] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated Gamma-synergin, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for AP1GBP1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: AP1GBP1
Alternate Names
AP1 gamma subunit binding protein 1, AP1 subunit gamma-binding protein 1, FLJ34482, Gamma-synergin, MGC104959, synergin gamma, synergin, gamma, SYNGAP1GBP1adaptor-related protein complex 1 gamma subunit-binding protein 1
Gene Symbol
SYNRG
UniProt
Additional AP1GBP1 Products
Product Documents for AP1GBP1 Antibody - BSA Free
Product Specific Notices for AP1GBP1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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