ANKRD15 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-03345
![Western Blot: ANKRD15 Antibody [NBP1-03345]](https://resources.bio-techne.com/images/products/ANKRD15-Antibody-Western-Blot-NBP1-03345-img0003.jpg "Western Blot: ANKRD15 Antibody [NBP1-03345]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1302 and 1352 of human KN motif and ankyrin repeat domains 1 using the numbering given in entry NP_055973.2 (GeneID 23189).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ANKRD15 Antibody - BSA Free
Western Blot: ANKRD15 Antibody [NBP1-03345]
Western Blot: ANKRD15 Antibody [NBP1-03345] - Detection of Human KANK1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KANK1 antibody NBP1-03345 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Western Blot: ANKRD15 Antibody [NBP1-03345]](https://resources.bio-techne.com/images/products/ANKRD15-Antibody-Western-Blot-NBP1-03345-img0001.jpg "Western Blot: ANKRD15 Antibody [NBP1-03345]")
Western Blot: ANKRD15 Antibody [NBP1-03345]
Western Blot: ANKRD15 Antibody [NBP1-03345] - Detection of Human KANK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- KANK1 antibody NBP1-03345 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. KANK1 was also immunoprecipitated by another rabbit anti-KANK1 antibody which recognizes an upstream epitope.
ANKRD15 Antibody [NBP1-03345] - Detection of human KANK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KANK1 antibody NBP1-03345 (lot NBP1-03345-2) used for IP at 6 ug per reaction. KANK1 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-03345-1) and another rabbit anti-KANK1 antibody. For blotting immunoprecipitated KANK1, NBP1-03345 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Applications for ANKRD15 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ANKRD15
Alternate Names
ANKRD15ankyrin repeat domain 15, Ankyrin repeat domain-containing protein 15, KANKDKFZp451G231, KIAA0172MGC43128, Kidney ankyrin repeat-containing protein, KN motif and ankyrin repeat domain-containing protein 1, KN motif and ankyrin repeat domains 1
Entrez Gene IDs
23189 (Human)
Gene Symbol
KANK1
UniProt
Additional ANKRD15 Products
Product Documents for ANKRD15 Antibody - BSA Free
Product Specific Notices for ANKRD15 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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