Amphiregulin Antibody (31221) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # FAB262CL1

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

MAB262

Allophycocyanin

FAB262A

Biotin

FAB262B

DyLight 350

FAB262D

DyLight 405

FAB262E

DyLight 488

FAB262K

DyLight 550

FAB262L

DyLight 594

FAB262M

DyLight 650

FAB262W

DyLight 680

FAB262Y

DyLight 755

FAB262Z

FITC

FAB262F

HRP

FAB262H

Janelia Fluor 525

FAB262JF525

Janelia Fluor 549

FAB262I

Janelia Fluor 646

FAB262J

Janelia Fluor 669

FAB262JF669

mFluor Violet 450 SE

FAB262MFV450

mFluor Violet 500 SE

FAB262MFV500

mFluor Violet 610 SE

FAB262MFV610

PE

FAB262P

PerCP

FAB262C

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), Neutralization

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ Clone # 31221

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Amphiregulin Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Amphiregulin

Specificity

Detects human Amphiregulin (AR) in Western blots. In this format, this antibody shows less than 5% cross-reactivity with recombinant human HB-EGF.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

CoraFluor(TM) 1, amine reactive

CoraFluor(TM) 1, thiol reactive

For more information, please see our CoraFluor(TM) TR-FRET technology flyer.

Scientific Data Images for Amphiregulin Antibody (31221) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for Amphiregulin Antibody (31221) [CoraFluor™ 1]

Application

Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Amphiregulin

The protein encoded by the Amphiregulin gene is a member of the epidermal growth factor family. It is an autocrine growth factor aswell as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) andtransforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote thegrowth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encodedprotein is associated with a psoriasis-like skin phenotype. (provided by RefSeq)

Alternate Names

AREG

Gene Symbol

AREG

Additional Amphiregulin Products

Product Documents for Amphiregulin Antibody (31221) [CoraFluor™ 1]

Product Datasheets

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COA

SDS

Product Specific Notices for Amphiregulin Antibody (31221) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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