alpha Tubulin [ac Lys40] Antibody (6-11B-1)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-567
Discontinued Product
NB600-567 has been discontinued.
View all alpha Tubulin products.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Amphibian, Bovine, Canine, Chicken, Hamster, Invertebrate, Parasite, Plant, Primate, Protozoa
Cited:
Human, Rat
Applications
Validated:
Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Dot Blot, Radioimmunoassay, Electron Microscopy
Cited:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 6-11B-1
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
This alpha Tubulin [ac Lys40] Antibody (6-11B-1) was developed against acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin)
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Modification
ac Lys40
Localization
Centrosomal.
Specificity
This alpha Tubulin [ac Lys40] Antibody (6-11B-1) recognizes an epitope located on the alpha 3 isoform of Chlamydomonas axonemal alpha-tubulin, within four residues of Lys-40 when this amino acid is acetylated
Marker
Microtubule Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for alpha Tubulin [ac Lys40] Antibody (6-11B-1)
Immunohistochemistry: alpha Tubulin [ac Lys40] Antibody (6-11B-1) [NB600-567] - Nerve pathways in the embryonic zebrafish brain and spinal cord revealed by alpha Tubulin (ac Lys40) Antibody (6-11B-1). Immunoperoxidase staining of a whole-mount embyro at 24 hours of development.
Western Blot: alpha Tubulin [ac Lys40] Antibody (6-11B-1) [NB600-567] - Western Blot validation of monoclonal anti-Acetylated Tubulin antibody clone: 6-11B-1 at 1:2000 dilution with goat anti-Mouse IgG-Peroxidase secondary antibodychemiluminescent substrate development. The lysates employed were from cell lines: 1. HeLa, 2. U87, 3. COS7, 4. P19, 5. RAT2, 6. CHO, 7. MDBK and 8. MDCK Alpha tubulin molecular weight: 50 kDa.
Immunocytochemistry/Immunofluorescence: alpha Tubulin [ac Lys40] Antibody (6-11B-1) [NB600-567] - HeLa cells were fixed with 4% paraformaldehyde followed permeabilization with 0.5% Triton X-100. Fixed cells were stained with 1:2,000 monoclonal Tubulin, Acetylated antibody Clone: 6-11B-1 . The antibody was detected using goat anti-Mouse IgG, Cy3 conjugate followed by counterstaining with DAPI (blue).
Applications for alpha Tubulin [ac Lys40] Antibody (6-11B-1)
Application
Recommended Usage
Dot Blot
1:100-1:2000
ELISA
1:100-1:2000
Electron Microscopy
1:10-1:500
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Immunohistochemistry
1:10-1:500
Radioimmunoassay
1:100-1:2000
Western Blot
1:2000
Reviewed Applications
Read 1 review rated 5 using NB600-567 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Preservative
0.09% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: alpha Tubulin
Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).
References
1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T., . . . Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921
2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718
Long Name
Tubulin Alpha 1a
Alternate Names
Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1, alpha tubulin loading control, alpha tubulin monoclonal, TUBA1 monoclonal, tubulin monoclonal
Gene Symbol
TUBA1A
Additional alpha Tubulin Products
Product Documents for alpha Tubulin [ac Lys40] Antibody (6-11B-1)
Product Specific Notices for alpha Tubulin [ac Lys40] Antibody (6-11B-1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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