Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 189508

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all alpha-Fetoprotein/AFP Antibodies »

Product Specifications

Immunogen

Human umbilical cord serum-derived alpha -Fetoprotein/AFP

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Human alpha-Fetoprotein MAb (Clone 189508)

Scientific Data Images for alpha-Fetoprotein/AFP Antibody (189508) - Azide and BSA Free

Knockout Validated: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692]

Knockout Validated: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692]

Knockout Validated: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692] - Western blot shows lysates of HepG2 human liver carcinoma cells parental cell line and AFP knockout (KO) HepG2 cell line. PVDF membrane was probed with 0.05 ug/mL of Mouse Anti-Human AFP Monoclonal Antibody (Catalog # MAB13692) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for AFP at approximately 74 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in the knockout HepG2 cell line. This experiment was conducted under reducing conditions.
Western Blot: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692]

Western Blot: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692]

Western Blot: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692] - Western blot shows lysates of undifferentiated and differentiated IBJ6 human induced pluripotent stem cells, and HepG2 and Hep3B cell lines. PVDF membrane was probed with 0.05 ug/mL of Mouse Anti-Human AFP Monoclonal Antibody (Catalog # MAB13692) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for AFP in the differentiated IBJ6 cells at approximately 74 kDa (as indicated), the HepG2 cells and Hep3B cells. This experiment was conducted under reducing conditions.
Simple Western: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692]

Simple Western: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692]

Simple Western: alpha-Fetoprotein/AFP Antibody (189508) [MAB13692] - Lane view shows a specific band for Fetoprotein-AFP in 0.2 mg/mL of HepG2 and HepB3 lysate. Antibody at 5 ug/mL. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for alpha-Fetoprotein/AFP Antibody (189508) - Azide and BSA Free

Application
Recommended Usage

Simple Western

10 ug/ml

Western Blot

0.1 - 0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha-Fetoprotein/AFP

Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Alternate Names

AFP, DSCAM2

Gene Symbol

AFP

Additional alpha-Fetoprotein/AFP Products

Product Documents for alpha-Fetoprotein/AFP Antibody (189508) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha-Fetoprotein/AFP Antibody (189508) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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