Acrolein Antibody (10A10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59359

Novus Biologicals, part of Bio-Techne
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NBP2-59359-25ug
NBP2-59359
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Non-species specific

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10A10

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Acrolein Antibodies »

Product Specifications

Immunogen

Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for Acrolein modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Acrolein Antibody (10A10) - BSA Free (NBP2-59359) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-Acrolein Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Acrolein Antibody (10A10) - BSA Free

Western Blot: Acrolein Antibody (10A10) [NBP2-59359]

Western Blot: Acrolein Antibody (10A10) [NBP2-59359]

Western Blot: Acrolein Antibody (10A10) [NBP2-59359] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10 (NBP2-59359). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59359) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.
Immunocytochemistry/ Immunofluorescence: Acrolein Antibody (10A10) [NBP2-59359]

Immunocytochemistry/ Immunofluorescence: Acrolein Antibody (10A10) [NBP2-59359]

Immunocytochemistry/Immunofluorescence: Acrolein Antibody (10A10) [NBP2-59359] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Immunohistochemistry: Acrolein Antibody (10A10) [NBP2-59359]

Immunohistochemistry: Acrolein Antibody (10A10) [NBP2-59359]

Immunohistochemistry: Acrolein Antibody (10A10) [NBP2-59359] - Immunohistochemistry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Adrenal Carcinoma. Species: Human. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.
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Applications for Acrolein Antibody (10A10) - BSA Free

Application
Recommended Usage

ELISA

1:1000

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Acrolein

Alternate Names

Acrylic amide; Ethylene Carboxamide 2-Propenamide; 2-Propen-1-one; 2-propenal; Acraldehyde; Acrolein; Acrylic aldehyde

Additional Acrolein Products

Product Documents for Acrolein Antibody (10A10) - BSA Free

Product Datasheets

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Product Specific Notices for Acrolein Antibody (10A10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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