A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59941
![Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2)BSA Free [NBP2-59941]](https://resources.bio-techne.com/images/products/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Western-Blot-NBP2-59941-img0007.jpg "Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2)BSA Free [NBP2-59941]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Bacteria
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SS 3A5-E2
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
GST fused to A. aeolicus BPL/BioID2
Reactivity Notes
Aquifex aeolicus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free
Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2)BSA Free [NBP2-59941]
Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Analysis in total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line stably expressing BioID2-TorsinA delta E302/3 without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BPL R40G/BioID2 with SS 3A5-E2 antibody. The predicted molecular weight of BioID2-Torsin A Delta E302/3 fusion protein is 65 kDa![Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free [NBP2-59941]](https://resources.bio-techne.com/images/products/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Immunocytochemistry-Immunofluorescence-NBP2-59941-img0005.jpg "Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free [NBP2-59941]")
Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free [NBP2-59941]
Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - A) Parental HEK293T or (B) monoclonal HEK293T cell line with BioID2 knocked-in into at least one LMNA gene alleles (resulting in BioID2 Lamin A/C fusion protein) were immunofluorescently labelled with anti-BPL R40G/BioID2 SS 3A5-E2 monoclonal antibody (green). The nuclei were visualized with Hoechst.![Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free [NBP2-59941]](https://resources.bio-techne.com/images/products/A-aeolicus-BPL-BioID2-Antibody-SS-3A5-E2-Immunocytochemistry-Immunofluorescence-NBP2-59941-img0002.jpg "Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free [NBP2-59941]")
Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free [NBP2-59941]
Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Staining of HeLa polyclonal cell line expressing BioID2 tagged to TorsinA delta E302/3 with SS 3A5-E2 monoclonal antibody. Cells were fixed in (A) cold methanol or (B) 4% PFA and stained with SS anti-BPL R40G/BioID2 3A5-E2 antibody (in green). The nuclei were counter-stained with Hoechst.Applications for A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Western Blot
1:100 - 1-2000
Application Notes
Positive Control: Cells overexpressing A. aeolicus BPL R40G (BioID2) construct.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Product Documents for A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free
Product Specific Notices for A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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