5'-Nucleotidase/CD73 Antibody (AD2) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22353

Discontinued Product
NBP2-22353 has been discontinued. View all 5'-Nucleotidase/CD73 products.

Key Product Details

Species Reactivity

Human, Primate

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # AD2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 5'-Nucleotidase/CD73 Antibodies »

Product Specifications

Immunogen

NBP2-22353, clone AD2 recognizes CD73, a 70 kDa GPI-anchored 5'-nucleotidase.

Reactivity Notes

Human.

Specificity

NBP2-22353 AD2 recognizes CD73, a 70 kDa GPI-anchored 5'-nucleotidase expressed predominantly on T and B cell subsets, follicular dendritic cells and endothelial cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for 5'-Nucleotidase/CD73 Antibody (AD2) [FITC]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 4 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. > 95% purity by SDS-PAGE

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Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

Phosphate buffered saline (PBS), pH 7.4

Preservative

15mM Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: 5'-Nucleotidase/CD73

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Gene Symbol

NT5E

Additional 5'-Nucleotidase/CD73 Products

Product Documents for 5'-Nucleotidase/CD73 Antibody (AD2) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for 5'-Nucleotidase/CD73 Antibody (AD2) [FITC]

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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