5-MethylCytosine Antibody (RM231)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61470
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Vertebrate
Applications
Immunohistochemistry, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Methylated DNA Immunoprecipitation, Dot Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM231 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
BSA-conjugated 5-MethylCytosine
Specificity
This antibody reacts to 5-MethylCytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit 5-MethylCytosine Antibody (RM231) (NBP2-61470) is a recombinant monoclonal antibody validated for use in IHC, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for 5-MethylCytosine Antibody (RM231)
Flow Cytometry: 5-MethylCytosine Antibody (RM231) [NBP2-61470] -
Flow Cytometry: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - Flow Cytometry analysis of 5-MethylCytosine expression in HEK293 cells using NBP2-61470. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with NBP2-61470 (Blue) or with a negative control antibody
ELISA: 5-MethylCytosine Antibody (RM231) [NBP2-61470] -
ELISA: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - Direct ELISA of HeLa cell genomic DNA using NBP2-61470. The plate was directly coated with different concentrations of genomic DNA isolated from HeLa cells. NBP2-61470 was used as the primary antibody, and a HRP conjugated Anti-Rabbit IgG as the secondary antibody.
ELISA: 5-MethylCytosine Antibody (RM231) [NBP2-61470] -
ELISA: 5-MethylCytosine Antibody (RM231) [NBP2-61470] - ELISA of single stranded DNA using NBP2-61470. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of NBP2-61470 was used as the primary antibody, and an alkaline phosphatase conjugated anti-Rabbit IgG as the secondary antibody.Applications for 5-MethylCytosine Antibody (RM231)
Application
Recommended Usage
Dot Blot
0.5 ug/ml - 2 ug/ml
ELISA
0.1 ug/ml - 1 ug/ml
Flow Cytometry
0.5 ug/ml-2 ug/ml
Immunocytochemistry/ Immunofluorescence
0.5 ug/ml - 2 ug/ml
Methylated DNA Immunoprecipitation
0.2 ug/ml - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: 5-MethylCytosine
Alternate Names
5-mC, 5-Methyl Cytosine, Cytosine (5-Methyl)
Additional 5-MethylCytosine Products
Product Documents for 5-MethylCytosine Antibody (RM231)
Product Specific Notices for 5-MethylCytosine Antibody (RM231)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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