5-hmC Antibody (RM236)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61469
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Vertebrate
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Methylated DNA Immunoprecipitation, Dot Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM236 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
BSA-conjugated 5-hmC
Specificity
This antibody reacts to 5-hmC in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit 5-hmC Antibody (RM236) (NBP2-61469) is a recombinant monoclonal antibody validated for use in IHC, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for 5-hmC Antibody (RM236)
Immunohistochemistry: 5-hmC Antibody (RM236) [NBP2-61469] -
Immunohistochemistry: 5-hmC Antibody (RM236) [NBP2-61469] - Immunohistochemical staining of formalin fixed and paraffin embedded Human brain tissue sections, using NBP2-61469
Flow Cytometry: 5-hmC Antibody (RM236) [NBP2-61469] -
Flow Cytometry: 5-hmC Antibody (RM236) [NBP2-61469] - Flow Cytometry analysis of 5-hmC expression in HEK293 cells using NBP2-61470. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with NBP2-61469 (Blue) or with a negative control antibody (red)
Methylated DNA Immunoprecipitation: 5-hmC Antibody (RM236) [NBP2-61469] -
Methylated DNA Immunoprecipitation: 5-hmC Antibody (RM236) [NBP2-61469] - hMeDIP was performed using Anti-5-hmC antibody (NBP2-61469) at a 10:1 DNA: Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1ug of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.
Applications for 5-hmC Antibody (RM236)
Application
Recommended Usage
Dot Blot
0.2 ug/ml - 1 ug/ml
ELISA
0.1 ug/ml - 1 ug/ml
Flow Cytometry
1 ug/ml-5 ug/ml
Immunocytochemistry/ Immunofluorescence
0.5 ug/ml - 2 ug/ml
Immunohistochemistry
0.1 ug/ml - 1 ug/ml
Methylated DNA Immunoprecipitation
0.2 ug/ml - 2ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: 5-hmC
Alternate Names
5'-hmC, 5-Hydroxymethylcytosine
Additional 5-hmC Products
Product Documents for 5-hmC Antibody (RM236)
Product Specific Notices for 5-hmC Antibody (RM236)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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