The HT PARP In Vivo Pharmacodynamic ELISA Kit II (2nd Gen), 4520B-096-K, has been transitioned and replaced by a new version, 4520C-096-K, which is now available.
Key Product Details
Features:
Monitors in vivo PARP activity within cells based on levels of PAR.
Key Benefits:
Pre-coated 96 well capture antibody plates
High signal to noise ratio
Detection sensitivity of 10 pg/mL of PAR
Broad linear dynamic range to 1,000 pg/mL
Reduced inter-assay variability
Validated assay that measures drug action on PARP in both in vivo and in vitro settings
Summary for HT PARP in vivo Pharmacodynamic ELISA Kit II
Principle of the Assay
A monoclonal antibody specific for PAR has been pre-coated onto a microplate to capture cellular PAR and PAR attached to proteins. Incubation with a PAR Monoclonal Biotinylated Detecting Antibody, followed by addition of Streptavidin-HRP and a chemiluminescent HRP substrate yields relative light units (RLU) that directly correlate with the amount of cellular PAR.
Product Specifications
Quantitation of PAR in tissue lysates and culture cells.
Monitoring the efficacy of PARP inhibitors on PAR formation in vivo.
Verifying observations of enhanced cancer cell cytotoxicity arising from PARP inhibitor/anticancer drug combination therapy.
Facilitating development of PARP and PARG targeted therapeutics.
Kit Contents for HT PARP in vivo Pharmacodynamic ELISA Kit II
PAR Standard, 25 pg/µl
WIL2-NS Cell Lysate Control Standard, Low
WIL2-NS Cell Lysate Control Standard, Medium
WIL2-NS Cell Lysate Control Standard, High
PAR Monoclonal Biotinylated Detecting Antibody
Streptavidin-HRP
DNase I, 2 Units/µl
PARP Sample Buffer
PARP Cell Lysis Reagent
100X Magnesium Cation
PARP Antibody Diluent
20% SDS
Pre-coated white 96-stripwell plate and 4 plate sealers
PARP PeroxyGlow A
PARP PeroxyGlow B
Product Specifications for HT PARP in vivo Pharmacodynamic ELISA Kit II
Species
Multi-species
Scientific Data Examples for HT PARP in vivo Pharmacodynamic ELISA Kit II
Affect of PJ34 PARP Inhibitor
Sample processing can potentially generate artifactual PAR synthesis during resuspension and incubation in Cell Lysis Buffer. Methods to mitigate this effect should be carefully considered as part of the experimental design. (e.g. The addition of a PARP inhibitor to the Cell Lysis Buffer such as PJ 34 Hydrochloride (Tocris™, Catalog #3255) can help mitigate artifactual PAR synthesis).
Preparation & Storage
Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
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