Caspase-4 Activity Assay Kit (Colorimetric)

Name: Caspase-4 Activity Assay Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-50231

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50231

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell and tissue samples

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Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

Caspase 4, also known as Ich-2(ICE and CED-3 homolog 2), also known as TX and ICErel-II, can interact with Apaf-1 and participate in caspase 3 activation caused by cytochrome c. Caspase 4 can also interact with caspase 14. This caspase 4 activity assay kit is based on caspase 4 that can catalyze the substrate Ac-LEVD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 4 can be calculated by determining the absorbance at 405 nm.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Caspase-4

Caspase 4 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.

Alternate Names

CASP4, Caspase4, ICE(rel)II, ICH-2, Mih1/TX, TX

Gene Symbol

CASP4

Additional Caspase-4 Products

Product Documents for Caspase-4 Activity Assay Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Caspase-4 Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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