Calcein AM
R&D Systems, part of Bio-Techne | Catalog # 4892-010-01
Cell permeable dye used to evaluate cell viability
Key Product Details
Features
Calcein AM is a cell-permeant dye that can be used to determine cell viability and/or cytotoxicity in most eukaryotic cells.
Product Summary for Calcein AM
What is Calcein AM?
Calcein AM (structure A) is a non-fluorescent, hydrophilic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein (structure B), a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates can be stained and quantified in less than two hours.
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Product Documents for Calcein AM
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