Biotin-NAD+

Name: Biotin-NAD+
Brand: Tocris Bioscience
SKU: 6573

Tocris Bioscience, part of Bio-Techne | Catalog # 6573

Citations (9)

COA / SDS

Biotinylated-NAD+; substrate for ADP-ribosylation

Key Product Details

Description:	Biotinylated-NAD⁺; substrate for ADP-ribosylation
Chemical Name:	β-Nicotinamide-N⁶-[2-[[6-[biotinyl]amino]hexyl]amino]-2-oxoethyl]adenine dinucleotide
Molecular Weight:	1045.95

View Full Technical Details for Biotin-NAD+

Technical Data
Citations

Biological Activity for Biotin-NAD+

Biotin-NAD+ is a provides a convenient non-isotopic alternative to radiolabeled NAD⁺ for determination of IC₅₀ values for candidate PARP inhibitors and studies requiring this substrate. Biotinylated-NAD⁺ allows an indirect measure of PARP activity when biotin incorporation is detected using a conjugated-streptavidin detection system. Acts as a substrate for ADP-ribosylation. Can be used to label and purify biotinyl-ADP ribosylated proteins.

This product is a replacement for R&D Systems product 4670-500-01.

131 μg is supplied as 500 μl of a 0.25 mM solution in water.

Technical Data for Biotin-NAD+

M. Wt	1045.95
Formula	C₃₉H₅₇N₁₁O₁₇P₂S
Storage	Store at -80°C
CAS Number	146385-37-7
InChi Key	MIIADCCJYSONEI-RNWYXDGLSA-N
Smiles	[H][C@]12CS[C@H]([C@]1(NC(N2)=O)[H])CCCCC(NCCCCCCNC(CNC3=C(N=CN4[C@@H]5O[C@@H]([C@H]([C@H]5O)O)COP(O)(OP(OC[C@H]6O[C@@H]([N+]7=CC=CC(C(N)=O)=C7)[C@H](O)[C@@H]6O)([O-])=O)=O)C4=NC=N3)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Biotin-NAD+

Solubility

Soluble in water (supplied pre-dissolved at a concentration of 0.25mM)

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References for Biotin-NAD+

References are publications that support the biological activity of the product.

Yang Antitumor activity of a pyrrole-imidazole polyamide. Proc.Natl.Acad.Sci.USA 2013 PMID: 23319609
Yang Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9 Mol. Cell 2017 PMID: 28525742
Schuster The Hsp90 machinery facilitates the transport of diphtheria toxin into human cells. Sci.Rep. 2017 PMID: 28377614
Zhang Purification of a nitric oxide-stimulated ADP-ribosylated protein using biotinylated beta-nicotinamide adenine dinucleotide. Biochemistry 1993 PMID: 8443164