Recombinant Rhesus Macaque/Cynomolgus IFN-alpha Protein
R&D Systems, part of Bio-Techne | Catalog # 14110-1
Key Product Details
- R&D Systems E. coli-derived Recombinant Rhesus Macaque/Cynomolgus IFN-alpha Protein (14110-1)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Source
E. coli-derived cynomolgus monkey IFN-alpha protein
Purity
>98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass
19.5 kDa
Activity
Interferon was titrated with the use of the cytopathic effect inhibition assay as listed. The protein has similar activity to Human IFN Alpha 2 on human, rhesus and bovine cells.
Bovine (MDBK/VSV) – performed as described [Rubinstein et al. (1981) J. Virol. 37(2):755] The EC50 for interferon in this assay is ~5 U/ml.
Human (A549/EMCV) – performed as described [Budd et al. (1985) Canc. Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml.
Formulation, Preparation and Storage
14110-1
| Formulation | Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Background: IFN-alpha
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Long Name
Interferon alpha
Alternate Names
IFNalpha, LeIF D
Entrez Gene IDs
3440 (Human)
Gene Symbol
IFNA2
UniProt
Additional IFN-alpha Products
Product Documents for Recombinant Rhesus Macaque/Cynomolgus IFN-alpha Protein
Product Specific Notices for Recombinant Rhesus Macaque/Cynomolgus IFN-alpha Protein
For research use only
Loading...
Loading...