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Recombinant Mouse MEGF9 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10598-ME

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10598-ME-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse MEGF9 protein
Gly35-Asn512, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

N-terminal Sequence Analysis

Gly35

Predicted Molecular Mass

51 kDa

SDS-PAGE

101-160 kDa, under reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Mouse MEGF9 His-tag, immobilized at 0.15 μg/mL on a 96 well plate, is able to significantly inhibit neurite outgrowth.

Scientific Data Images for Recombinant Mouse MEGF9 His-tag Protein, CF

Recombinant Mouse MEGF9 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse MEGF9 His-tag (Catalog # 10598-ME) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 101-160 kDa.

Formulation, Preparation and Storage

10598-ME
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEGF9

MEGF9 (Multiple EGF‑like domains protein 9; also EGF‑like protein 5) is a transmembrane glycoprotein with multiple EGF-like repeats (1). It is predominantly expressed in the developing and adult central nervous system and peripheral nervous system. Immunohistochemical studies showed that MEGF9 is expressed in Purkinje cells of the cerebellum and in glial cells of the peripheral nervous system. In non-neuronal tissues, MEGF9 is expressed in epidermal layer of skin, papillae of the tongue and epithelium of the gastrointestinal tract. MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration (1). Mature mouse MEGF9 is 566 amino acids (aa) in length. It is a single span type I transmembrane protein that contains a 478 aa extracellular domain (ECD) that possesses a lengthy Pro‑rich region. N-terminal region of MEGF9 includes several potential O-glycosylation sites followed by five EGF-like domains which are highly homologous to the short arms of laminins. Following one single pass transmembrane domain, a highly conserved short intracellular domain with potential phosphorylation sites is present (1, 2). Within the ECD, mouse MEGF9 shares 75% and 92% aa sequence identity with mouse and rat MEGF9, respectively.

References

  1. Brandt-Bohne, U. et al. (2007) Biochem. J. 401:447.
  2. Heller, B. et al. (2014) J. Cell. Biol. 204:1219.

Long Name

Multiple EGF-like-domains 9

Alternate Names

EGFL5

Entrez Gene IDs

1955 (Human); 230316 (Mouse); 313270 (Rat)

Gene Symbol

MEGF9

UniProt

Additional MEGF9 Products

Product Documents for Recombinant Mouse MEGF9 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse MEGF9 His-tag Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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