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Recombinant Human BMP-8a Protein, CF
Catalog # 1073-BP/CF | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Accession #
Source
E. coli
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
E. coli-derived human BMP-8a protein
Ala264-His402 & Val265-His402
Ala264-His402 & Val265-His402
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala264 & Val265
Predicted Molecular Mass
15.8 kDa (monomer)
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human BMP‑8a is present at 1 μg/mL, the concentration of recombinant human BMPR‑1A Fc Chimera that produces 50% of the optimal binding response is approximately 1.5‑6 μg/mL.
When Recombinant Human BMP‑8a is present at 1 μg/mL, the concentration of recombinant human BMPR‑1A Fc Chimera that produces 50% of the optimal binding response is approximately 1.5‑6 μg/mL.
Formulation, Preparation and Storage
1073-BP/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: BMP-8a
BMP-8, also known as osteogenic protein 2 (OP-2), was first isolated from a hippocampal library in a screen to identify relatives of BMP-7 (OP-1) (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs (2). BMPs play roles in regulating growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal, epithelial, hematopoetic, and neuronal cells.
There exist two highly related and closely linked genes, designated BMP-8a and -8b in mice and humans. For humans, the protein products of these two genes share 98% amino acid (aa) sequence identity in their pro- and mature regions. However in the mouse, the two proteins share 89% and 76% aa sequence homology in their pro- and mature regions, respectively (3). Mature human BMP-8a shares 91% and 70% aa sequence identity with mouse BMP-8a and -8b, respectively. Human BMP‑8a is synthesized as a large precursor protein that is cleaved at a dibasic cleavage site (RTPR) between aa residues 263 and 264 to release a 139 aa carboxy‑terminal domain. Expression patterns of the BMP-8 genes indicate that they regulate aspects of cell proliferation and/or differentiation during spermatogenesis and formation of the placenta (3). BMP-8 is also highly expressed in osteosarcomas (4).
References
- Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
- Canalis, E. et al. (2003) Endocrine Rev. 24:218.
- Zhao, G-Q. et al. (1996) Mech. Dev. 57:159.
- Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.
Long Name
Bone Morphogenetic Protein 8a
Alternate Names
Op2, Osteogenic Protein 2
Gene Symbol
BMP8A
UniProt
Product Documents for Recombinant Human BMP-8a Protein, CF
Product Specific Notices for Recombinant Human BMP-8a Protein, CF
For research use only
Citations for Recombinant Human BMP-8a Protein, CF (9)
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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human BMP-8a Protein, CF include:
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Species: Mouse
Sample Types: Whole Cells
Applications: BioassayX Wang et al. (2021-02-19), Formative pluripotent stem cells show features of epiblast cells poised for gastrulation Cell Research, 2021-00(0).
PMID: 33608671 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayAD Senft et al. (2019-03-06), Genetic dissection of Nodal and Bmp signalling requirements during primordial germ cell development in mouse Nat Commun, 2019-010(1):1089.
PMID: 30842446 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayAD Senft et al. (2018-08-21), Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming Cell Rep, 2018-024(8):1977-1985.e7.
PMID: 30134160 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayD Jung et al. (2017-06-12), In vitro differentiation of human embryonic stem cells into ovarian follicle-like cells Nat Commun, 2017-08(0):15680.
PMID: 28604658 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayDominguez et al. (2014-09-22), Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies. Sci Rep, 2014-04(0):6432.
PMID: 25242416 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassaySharma A et al. (2014-03-21), Brown fat determination and development from muscle precursor cells by novel action of bone morphogenetic protein 6. PLoS ONE, 2014-09(3):e92608.
PMID: 24658703 -
Species: Human
Sample Types: Whole Cells
Applications: Cell CultureGoyal et al. (2013-12-12), Generation of human induced pluripotent stem cells using epigenetic regulators reveals a germ cell-like identity in partially reprogrammed colonies. PLoS ONE, 2013-18(12):e82838.
PMID: 24349377 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayWilson TR et al. (2012-07-26), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-0487(7408):505-9.
PMID: 22763448 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGeens M et al. (2011-02-12), Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells. J. Assist. Reprod. Genet., 2011-00(0).
PMID: 21318592
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