Recombinant Human beta-NGF Protein, CF

Catalog # 256-GF/CF | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

CAA36832

Source

NS0

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human beta-NGF protein
Ser122-Ala241

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser122

Predicted Molecular Mass

13.5 kDa

SDS-PAGE

13 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 0.2-2 ng/mL.

Scientific Data Images for Recombinant Human beta-NGF Protein, CF

Recombinant Human beta-NGF Protein Bioactivity

Recombinant Human beta-NGF Protein Bioactivity

Recombinant Human beta-NGF (Catalog # 256-GF/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.2-2 ng/mL.
Recombinant Human beta-NGF Protein SDS-PAGE

Recombinant Human beta-NGF Protein SDS-PAGE

1 µg/lane of Recombinant Human beta-NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.

Formulation, Preparation and Storage

256-GF/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetic Acid.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-NGF

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and  gamma. Both the alpha and  gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta-NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta-NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta-NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Long Name

beta Nerve Growth Factor

Alternate Names

betaNGF, NGFB

Entrez Gene IDs

4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)

Gene Symbol

NGF

UniProt

CAA36832

Product Documents for Recombinant Human beta-NGF Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human beta-NGF Protein, CF

For research use only

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