Recombinant Human Activin C Protein, CF

Catalog # 1629-AC/CF | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

P55103

Source

HEK293

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Activin C protein
Gly237-Ser352

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly237

Predicted Molecular Mass

12.5 kDa (monomer)

SDS-PAGE

13 kDa, reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Activin RIIA Fc Chimera (CHO) (Catalog # 340-RC2) is immobilized at 2.5 µg/mL (100 µL/well), the concentration of Recombinant Human Activin C that produces 50% of the optimal binding response is approximately 6‑30 ng/mL.

Formulation, Preparation and Storage

1629-AC/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.

Background: Activin C

Activins and Inhibins are TGF‑ beta superfamily proteins that regulate a wide range of processes including mesoderm induction, reproductive system development and function, liver growth and regeneration, wound healing, and inflammation. Activins signal through heterodimeric receptor complexes composed of type I (Activin RIA or RIB) and type II (Activin RIIA or RIIB) transmembrane Ser/Thr kinases. There are four human Inhibin beta subunits ( betaA, betaB, betaC, and betaE) and a single Inhibin alpha subunit, each of which adopts a cysteine‑knot structure (1‑3). Activins are disulfide‑linked homodimers or heterodimers of beta subunits, while Inhibins contain the alpha subunit and betaA or  betaB. Human betaC consists of an 18 aa signal sequence, a 218 aa propeptide, and a 116 aa mature segment (4). Mature human betaC shares 51%, 53%, and 64% aa sequence identity with human betaA, betaB, and betaE, respectively. It shares 93% and 91% aa sequence identity with mouse and rat  betaC, respectively. The expression of betaC is restricted compared to the widespread distribution of betaA and betaB. Activin C is expressed as an approximately 20 kDa dimer predominantly by hepatocytes but also by multiple cell types in the male and female reproductive tracts, posterior pituitary and adrenal glands, and nociceptive afferent dorsal root ganglia neurons (5‑7). The betaC subunit regulates Activin induced effects in a variety of systems by forming intracellular dimers with the betaA subunit and impeding the release of Activins A and AB (8, 9). It also functions extracellularly by interfering with Activin A‑receptor interactions (6, 7, 10). betaC can additionally form heterodimers with the betaB or betaE subunits (9, 11, 12).

References

  1. Butler, C.M. et al. (2005) Cytokine Growth Factor Rev. 16:377.
  2. Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157.
  3. Walton, K.L. et al. (2012) Mol. Cell. Endocrinol. 359:2.
  4. Hötten, G. et al. (1995) Biochem. Biophys. Res. Commun. 206:608.
  5. Gold, E.J. et al. (2004) Mol. Cell. Endocrinol. 222:61.
  6. Wada, W. et al. (2004) Am. J. Physiol. Endocrinol. Metab. 287:E247.
  7. Liu, X.-J. et al. (2012) Brain 135:391.
  8. Mellor, S.L. et al. (2003) Endocrinology 144:4410.
  9. Mellor S.L. et al. (2000) J. Clin. Endocrinol. Metab. 85:4851.
  10. Gold, E. et al. (2009) Am. J. Pathol. 174:184.
  11. Vejda, S. et al. (2002) J. Mol. Endocrinol. 28:137.
  12. Wada, W. et al. (2005) Endocr. J. 52:169.

Alternate Names

INHBC

Entrez Gene IDs

3626 (Human); 16325 (Mouse)

Gene Symbol

INHBC

UniProt

P55103

Product Documents for Recombinant Human Activin C Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Activin C Protein, CF

For research use only

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