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Recombinant HCoV-OC43 Nucleocapsid His-tag Protein, CF

Catalog # 10709-CVB | R&D Systems, Inc. a Bio-Techne Brand
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10709-CVB-100

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived hcov-oc43 Nucleocapsid protein
Met1-Ile448, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Identity confirmed by mass spectrometry

Predicted Molecular Mass

50 kDa

SDS-PAGE

54-63 kDa, under reducing conditions.

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant HCoV-OC43 Nucleocapsid His-tag Protein, CF

Recombinant HCoV-OC43 Nucleocapsid His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant HCoV-OC43 Nucleocapsid His-tag Protein (Catalog # 10709-CVB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 41-63 kDa.

Formulation, Preparation and Storage

10709-CVB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nucleocapsid

HCoV-OC43, a virus first isolated in 1960's that accounts for ~ 20% of the common cold, belongs to a family of viruses known as coronaviruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1, 2). Other well-known human coronaviruses include three viruses that cause relatively mild respiratory disease: HCoV-229E, HCoV-HKU1 and HCov-NL63, plus three viruses that cause the Severe Acute Respiratory Syndrome (SARS-CoV), the Middle East Respirator Syndrome (MERS-CoV), and the global pandemic Covid-19 (SARS-CoV2). While the S, E and M proteins build up the viral envelop, the N protein is involved transcription, replication, and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (3, 4). The CoV-OC43 N protein is a ~50 kDa protein composed of two independent structural domains connected by a linker region. Both the N-terminal and the linker regions contain RNA binding domains, while the C-terminal region is responsible for the oligomerization of the N protein (5). The CoV‑OC43 N protein shares 64% amino acid sequence identity with CoV-HKU1 N protein. the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity. Cross activity of antibodies among different strains should be rigorously tested when designing serological diagnostic kits (6, 7).

References

  1. St-Jean, J.R. et al. (2004) J. Virol. 78:8824.
  2. Vabret, A. et al. (2003) Fr Clin Infect Dis. 36:985.
  3. Chang, C.K. et al. (2006) J. Biomed. Sci. 13:59.
  4. Hurst, K.R. et al. (2009) J. Virol. 83:7221.
  5. Huang, C.Y. et al. (2009) Protein Sci. 18:2209.
  6. Chan, K.H. et al. (2005) Clin Diagn Lab Immunol. 12:1317.
  7. Mourez, T. et al. (2007) J. Virol Methods. 139:175.

Long Name

Nucleocapsid Protein

Alternate Names

N Protein

Entrez Gene IDs

918763 (HCoV-229E); 3200423 (HCoV-HKU1); 2943504 (HCoV-NL63); 39105221 (HCoV-OC43); 14254601 (MERS-CoV); 1489678 (SARS-CoV); 43740575 (SARS-CoV-2)

Gene Symbol

N

UniProt

Product Documents for Recombinant HCoV-OC43 Nucleocapsid His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant HCoV-OC43 Nucleocapsid His-tag Protein, CF

For research use only

Reconstitution Calculator

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