Recombinant Mouse FGF-8 Protein

Name: Recombinant Mouse FGF-8 Protein
Brand: Novus Biologicals
SKU: NBP2-35033

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35033

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P37237

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all FGF-8 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 246 amino acids corresponding to FGF-8 Source: E. coli

Uniprot ID: P37237

Amino Acid Sequence: QVRSAAQKRG PGAGNPADTL GQGHEDRPFG QRSRAGKNFT NPAPNYPEEG SKEQRDSVLP KVTQRHVREQ SLVTDQLSRR LIRTYQLYSR TSGKHVQVLA NKRINAMAED GDPFAKLIVE TDTFGSRVRV RGAETGLYIC MNKKGKLIAK SNGKGKDCVF TEIVLENNYT ALQNAKYEGW YMAFTRKGRP RKGSKTRQHQ REVHFMKRLP RGHHTTEQSL RFEFLNYPPF TRSLRGSQRT WAPEPR

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of FGF-8 as determined by LAL method.

Predicted Molecular Mass

28.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

FGF-8 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 x 10^5 IU/mg in the presence of 10 ug/ml of heparin

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse FGF-8 Protein

SDS-PAGE: Recombinant Mouse FGF-8 Protein [NBP2-35033]

SDS-Page: Mouse FGF-8 Protein [NBP2-35033]

Formulation, Preparation and Storage

NBP2-35033

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, 500 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: FGF-8

Murine FGF-8 is a heparin binding growth factor belonging to the FGF family, which plays a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. Murine FGF-8 is first purified from an androgen-dependent mouse mammary carcinoma cell line as an androgen induces secretion. Cloning and analysis of the murine FGF8 gene revealed at least eight potential protein isoforms (FGF-8 a-h). Murine FGF-8a and b share 100 % amino acid identity with that in humans, and murine FGF-8e and f share 98 % amino acid identity with humans. None of the FGF-8 isoforms exhibited activity to FGFR1b, 2b, 3b, but FGFR2c, 3c and FGFR4 can be activated by several FGF-8 isoforms. FGF-8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration, and it is required for normal brain, eye, ear and limb development during embryogenesis.

Long Name

Fibroblast Growth Factor 8

Alternate Names

AIGF, FGF8, HBGF-8

Gene Symbol

FGF8

UniProt