GMP Human T Cell Media

T cell viability and growth are equivalent in fresh and pre-made media stored at 4 °C for at least 5 weeks. Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-7 (BT-007-GMP) and IL-15 (BT-015-GMP) cytokines. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. T cell viability (A), cell density (B), and fold expansion (C) after 7 days in culture are shown. Data is the average of 3 purified T cell donors with error bars ± SD.

T cell phenotype is maintained in fresh and pre-made media stored at 4 °C for at least 5 weeks. Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-7 (BT-007-GMP) and IL-15 (BT-015-GMP) cytokines. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. Frequency of CD4+ or CD8+ T cells (A), CD4+ T cell phenotype (B), and CD8+ phenotype (C) after 7 days in culture are shown. T cell phenotype is gated on CD45RA and CCR7. Data is the average of 3 purified T cell donors with error bars ± SD.

T cell viability and growth are equivalent in fresh and pre-made media stored at 4 °C for at least 5 weeks. Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-2 (BT-002-GMP) cytokine. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. T cell viability (A), cells density (B), and fold expansion (C) after 7 days in culture are shown. Data is the average of 3 purified T cell donors with error bars ± SD.

T cell phenotype is maintained in fresh and pre-made media stored at 4 °C for at least 5 weeks. Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-2 (BT-002-GMP) cytokine. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. Frequency of CD4+ or CD8+ T cells (A), CD4+ T cell phenotype (B), and CD8+ phenotype (C) after 7 days in culture are shown. T cell phenotype is gated on CD45RA and CCR7. Data is the average of 3 T cell donors with error bars ± SD.

T cells grown in a G-Rex® bioreactor show similar confluency and viability when supplemented with human AB serum or a xeno-free serum replacement. Purified CD4+ and CD8+ T cells from 3 healthy donors were cultured in a 6M G-Rex Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 5 ng/mL of Recombinant Human IL-7 GMP Protein (Catalog # BT-007-GMP), 2.5 ng/mL Recombinant Human IL-15 GMP Protein (Catalog # BT-015-GMP) and either 5% human AB serum or a xeno-free serum replacement. After 9 days in culture, cells were assessed for confluency and viability.

T cell fold expansion and viability with GMP Human T Cell Media are comparable to competitor media. Purified CD4+ and CD8+ T cells from 3 healthy donors were cultured in a 6M G-Rex Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 5 ng/mL of Recombinant Human IL-7 GMP Protein (Catalog # BT-007-GMP), 2.5 ng/mL Recombinant Human IL-15 GMP Protein (Catalog # BT-015-GMP) and 5% human AB serum, or a competitor media. After 9 days in culture, cells were assessed for fold expansion and viability.

T cell culture in GMP Human T Cell Media results in lower exhaustion marker expression. Purified CD4+ and CD8+ T cells from 3 healthy donors were cultured in a 6M G-Rex Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 5 ng/mL of Recombinant Human IL-7 GMP Protein (Catalog # BT-007-GMP), 2.5 ng/mL Recombinant Human IL-15 GMP Protein (Catalog # BT-015-GMP) and 5% human AB serum, or a competitor media. After 9 days in culture, cells were assessed for expression of exhaustion markers, PD-1 and Tim-3. Data is the average of 3 donors ± SD.

T cell memory phenotype markers after culture in GMP Human T Cell Media. Purified CD4+ and CD8+ T cells from 3 healthy donors were cultured in a 6M G-Rex Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 5 ng/mL of Recombinant Human IL-7 GMP Protein (Catalog # BT-007-GMP), 2.5 ng/mL Recombinant Human IL-15 GMP Protein (Catalog # BT-015-GMP) and 5% human AB serum, or a competitor media. After 9 days in culture, cells were assessed for expression of CD45RA and CCR7, as T_SCM (CD45RA+ CCR7+), T_CM (CD45RA- CCR7+), T_EM (CD45RA- CCR7-), and T_eff (CD45RA+ CCR7-). Data is the average of 3 donors ± SD.

GMP Human T Cell Media supports T cell editing with Lentivirus or the TcBuster Transposon system. Purified CD4+ and CD8+ T cells from 3 healthy donors were cultured in a 6M G-Rex Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 5 ng/mL of Recombinant Human IL-7 GMP Protein (Catalog # BT-007-GMP), 2.5 ng/mL Recombinant Human IL-15 GMP Protein (Catalog # BT-015-GMP) and 5% human AB serum. Activated T cells were transposed with the TcBuster transposon system or transduced with Lentivirus to express CD19CAR and eGFP. After 9 total days in culture, cells were assessed for expression of the inserted cargo (eGFP). Data is the average of 3 donors ± SD.