HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEU1 (, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEU1.

Applications for NEU-1/Sialidase-1 Antibody (OTI3F5)

Application

Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: NEU-1/Sialidase-1

The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Neu, a glycoprotein, undergoes transactivation upon heterodimerization with other EGF receptor family members. Neu heterodimerization with ErbB-3 recruits heregulin, which induces phosphoinositide (PI) 3-kinase activation. Activation of Neu potentiates tumor cell motility and protease secretion and invasion, and also modulates cell cycle checkpoint function, DNA repair and apoptotic responses. Amplification and/or overexpression of Neu occurs in 20-30% of breast carcinomas. Measurement of increased Neu expression can be a predictor of disease prognosis. Neu may also prove to be a promising target for therapeutic agents.

Long Name

N-acetyl-alpha-Neuraminidase 1

Alternate Names

NEU1, Sialidase-1

Gene Symbol

NEU1

Additional NEU-1/Sialidase-1 Products

Product Documents for NEU-1/Sialidase-1 Antibody (OTI3F5)

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for NEU-1/Sialidase-1 Antibody (OTI3F5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

NEU-1/Sialidase-1 Antibody (OTI3F5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44673

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all NEU-1/Sialidase-1 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for NEU-1/Sialidase-1 Antibody (OTI3F5)

Western Blot;NEU-1/Sialidase-1 Antibody (OTI3F5)[NBP3-44673]-

Applications for NEU-1/Sialidase-1 Antibody (OTI3F5)

Western Blot

Formulation, Preparation, and Storage

Purification

Reconstitution

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: NEU-1/Sialidase-1

Long Name

Alternate Names

Gene Symbol

Additional NEU-1/Sialidase-1 Products

Product Documents for NEU-1/Sialidase-1 Antibody (OTI3F5)

Product Datasheets

COA

Certificate of Analysis

Product Specific Notices for NEU-1/Sialidase-1 Antibody (OTI3F5)

CONTACT INFORMATION

保持关注