Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11429
![Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free [NBP3-11429] -](https://resources.bio-techne.com/images/products/nbp3-11429_mouse-monoclonal-macrophage-and-histiocytoma-marker-antibody-d11-azide-and-bsa-free-24120258193538.jpg "Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free [NBP3-11429] -")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse (Negative), Porcine (Negative), Rat (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # D11
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Membrane preparation from human hepatocytes
Reactivity Notes
Does not react with Mouse, Rat or Porcine.
Localization
Cell Surface & Cytoplasmic
Specificity
In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this monoclonal antibody and a standard CD68 monoclonal antibody showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This monoclonal antibody shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This monoclonal antibody does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.
Marker
Macrophage & Histiocytoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45278).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images
Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free [NBP3-11429] -
Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free [NBP3-11429] - Human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).![Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free [NBP3-11429] -](https://resources.bio-techne.com/images/products/nbp3-11429_mouse-monoclonal-macrophage-and-histiocytoma-marker-antibody-d11-azide-and-bsa-free-24120258203429.jpg "Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free [NBP3-11429] -")
Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free [NBP3-11429] -
Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) - Azide and BSA Free [NBP3-11429] - Human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).Applications
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Macrophage and Histiocytoma Marker
Additional Macrophage and Histiocytoma Marker Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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