Macrophage and Histiocytoma Marker Antibody (D11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45278

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse (Negative), Porcine (Negative), Rat (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # D11

Format

BSA Free

Concentration

0.2 mg/ml

View all Macrophage and Histiocytoma Marker Antibodies »

Product Specifications

Immunogen

Membrane preparation from human hepatocytes

Reactivity Notes

Does not react with Mouse, Rat or Porcine.

Localization

Cell Surface & Cytoplasmic

Specificity

In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this monoclonal antibody and a standard CD68 monoclonal antibody showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This monoclonal antibody shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This monoclonal antibody does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.

Marker

Macrophage & Histiocytoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% azide. Also available WITHOUT azide at 1.0 mg/ml. (NBP3-11429)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Macrophage and Histiocytoma Marker Antibody (D11) - BSA Free

Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) [NBP2-45278]

Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) [NBP2-45278]

Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) [NBP2-45278] - Human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).
Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) [NBP2-45278]

Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) [NBP2-45278]

Immunohistochemistry-Paraffin: Macrophage and Histiocytoma Marker Antibody (D11) [NBP2-45278] - Human Histiocytoma stained with Macrophage Monoclonal Antibody (D11).

Applications for Macrophage and Histiocytoma Marker Antibody (D11) - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Macrophage and Histiocytoma Marker

Additional Macrophage and Histiocytoma Marker Products

Product Documents for Macrophage and Histiocytoma Marker Antibody (D11) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Macrophage and Histiocytoma Marker Antibody (D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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