Detects human and rat SOX10. Epitope-mapping experiments indicated that clone 20B7 recognizes a determinant in the first 65 amino acids, which contains sequences unique to SOX10.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human/Rat SOX10 NorthernLights™ NL557-conjugated Antibody

Detection of SOX10 in Ntera2.

SOX10 was detected in immersion fixed Ntera2 cells using Mouse Anti-Human/Rat SOX10 NorthernLights™ NL557-conjugated Monoclonal Antibody (Catalog # NL2864R) at a final concentration of 1X (1:10 dilution) for 3 hours at room temperature (A). Cells were counterstained with DAPI (blue, B). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Rat SOX10 NorthernLights™ NL557-conjugated Antibody

Application

Recommended Usage

Immunocytochemistry

1:10 dilution
Sample: Immersion fixed NTera-2 human testicular embryonic carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied as a 10X solution of antibody in 0.5 mL PBS containing 0.1% sodium azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: SOX10

SOX10 belongs to the SOX family of transcription factors which display diverse roles during development. In the central nervous system, SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.

References

Lo, L. et al. (2002) Development 129:1553.

Long Name

Transcription Factor SOX10

Alternate Names

DOM, WS4

Entrez Gene IDs

6663 (Human)

Gene Symbol

SOX10

Additional SOX10 Products

Product Documents for Human/Rat SOX10 NorthernLights™ NL557-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Rat SOX10 NorthernLights™ NL557-conjugated Antibody

For research use only

Citations
Reviews

Human/Rat SOX10 NorthernLights™ NL557-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # NL2864R

Key Product Details

Species Reactivity

Validated:

Cited:

Applications