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Human A33 MAb (Clone 2867A)

Catalog # MAB30803 | R&D Systems, Inc. a Bio-Techne Brand
Recombinant Monoclonal Antibody.
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MAB30803-100
MAB30803-SP

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2867A

Product Summary for Human A33 MAb (Clone 2867A)

Immunogen

Mouse myeloma cell line NS0-derived recombinant human A33
Ile22-Val235
Accession # Q99795

Specificity

Detects human A33 in direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human A33 MAb (Clone 2867A)

Detection of A33 in HT‑29 Human Cell Line by Flow Cytometry.

HT-29 human colon carcinoma cell line was stained with Rabbit Anti-Human A33 Monoclonal Antibody (Catalog # MAB30803, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111).

Detection of Human A33 by Western Blot.

Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human A33 Monoclonal Antibody (Catalog # MAB30803) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for A33 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human A33 MAb (Clone 2867A)

Application
Recommended Usage

Flow Cytometry

0.25 µg/mL
Sample:  HT-29 human colon carcinoma cell line 

Western Blot

2 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A33

Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.

Long Name

Cell Surface A33 Antigen

Alternate Names

GPA33

Entrez Gene IDs

10223 (Human); 59290 (Mouse)

Gene Symbol

GPA33

UniProt

Product Documents for Human A33 MAb (Clone 2867A)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human A33 MAb (Clone 2867A)

For research use only

Reconstitution Calculator

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