登录/注册
Human A33 Antibody
Catalog # MAB30802 | R&D Systems, Inc. a Bio-Techne Brand
Discontinued Product
MAB30802 has been discontinued and is replaced by
MAB3080.
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1053809
Product Summary for Human A33 Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human A33
Ile22-Val235
Accession # Q99795
Ile22-Val235
Accession # Q99795
Specificity
Detects human A33 in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human A33 Antibody
Detection of Human A33 by Western Blot.
Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human A33 Monoclonal Antibody (Catalog # MAB30802) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for A33 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Human A33 by Simple WesternTM.
Simple Western lane view shows lysates of HT‑29 human colon adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for A33 at approximately 40 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human A33 Monoclonal Antibody (Catalog # MAB30802) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human A33 Antibody
Application
Recommended Usage
Simple Western
50 µg/mL
Sample: Lysates of HT-29 human colon adenocarcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line
Sample: Lysates of HT-29 human colon adenocarcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line
Western Blot
2 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line.
Sample: HT‑29 human colon adenocarcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: A33
Product Specific Notices for Human A33 Antibody
For research use only
Loading...
