May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?

Name: Caspase-8 Activity Assay Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-50233

In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Caspase-8 Activity Assay Kit (Colorimetric) New

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50233

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell and tissue samples

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Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

Caspase 8, also known as FLICE, MACH and Mch5 is self-activated or activated in response to granzyme B cleavage. Caspase 8 specifically recognizes IETD. Activated caspase 8 can not only cleave PARP, but also participate in the activation of other ICE family proteases, such as caspase 3 and caspase 7. This caspase 8 activity assay kit is based on caspase 8 that can catalyze the substrate Ac-IETD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 8 can be calculated by determining the absorbance at 405 nm.

Scientific Data Images for Caspase-8 Activity Assay Kit (Colorimetric)

Application: Caspase-8 Activity Assay Kit (Colorimetric) [NBP3-50233]

Standard Curve of Caspase-8 Activity Assay Kit (Colorimetric) [NBP3-50233]

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Caspase-8

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. The sequential activation of caspases plays a central role in the execution-phase of cell apoptosis, and Caspase 8 is the most upstream protease in the the caspase activation cascade.

Caspase 8 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 8 form.

Caspase 8 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Alternate Names

CASP8, Caspase8, Mch5

Gene Symbol

CASP8

Additional Caspase-8 Products

Product Documents for Caspase-8 Activity Assay Kit (Colorimetric)

Product Datasheets

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Protocols & Manuals

Download NBP3-50233

COA

Product Specific Notices for Caspase-8 Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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