Skip to main content

Cell Activation Kits

Cloudz Cell Activation Kit

The Sky Is The Limit

Cloudz is a customizable cell activation and expansion platform that reduces the complexity of cell processing. Cloudz microspheres are dissolvable, eliminating the extra handling step for clearing the reagent from your cultures.

What are Cloudz Reagents?

Cloudz reagents are microspheres composed of an alginate-based hydrogel. The microspheres are available in multiple sizes and can be functionalized with a variety of ligands including antibodies, proteins, and small molecules. Derivatized Cloudz reagents present solid phase bound ligands to cells to induce receptor crosslinking and intracellular signaling.

The alginate hydrogel quickly dissolves when exposed to the release buffer, allowing for gentle and efficient removal of the microspheres. This dissolving property also allows you to precisely terminate the Cloudz ligand signaling to your cells and reduces processing steps.

Cloudz Cell Activation Kits are optimized for use with ExCellerate™ media and R&D Systems™ cytokines.

Cloudz Expansion Protocol – A Simple 3-step Process

  • Add Cloudz to your cell culture.
  • Incubate cell culture with Cloudz for at least 2 days.
  • Add release buffer to dissolve Cloudz microspheres.

Expanded cells are ready for use, free of residual particulates.

Screening Cloudz

Screening cloudz ligandz cell

A Customizable Platform for Tuning Cell Expansion and Activation Protocols

Screening Cloudz enable you to discover the most effective ligands for your particular assay. They’re derivatized with streptavidin which means you have the flexibility to functionalize them with the biotinylated ligand of your choice. The sky is the limit!

Once you have identified your optimum ligands, we can manufacture a Cloudz kit at the scale you need.

Screening cloudz method

Unmatched Flexibility With Screening Cloudz

  • Target the cell surface molecules of your choice
  • Evaluate multiple antibody clones, protein constructs, or small molecule analogs
  • Optimize the number of ligands per microsphere
  • Optimize cocktails of Cloudz carrying different ligands
  • Evaluate stepwise cell activation by using different beads sequentially
  • Target inhibitory receptors with antagonistic ligands

Supporting Reagents and Services

Cloudz Kits for Immune Cell Activation and Expansion

Having a reliable and scalable cell expansion platform is critical for a growing manufacturing process. Cloudz kits are available in RUO and GMP-grade to support consistent and seamless process translations. If you do not see the ligands optimal to your process, contact our Custom Services team to develop a customized kit.

Description

Expansion Capacity

Formats

Ligands

Optimized For Use With

Cloudz Human T Cell Activation Kits

> 50-fold expansion of T cells from PBMCs or purified T cells in 10 days

RUO, GMP

Fully Humanized CD3 and CD28 Antibodies

ExCellerate Human T Cell Expansion Media, Recombinant Proteins, GMP Proteins

Cloudz Human Treg Cell Activation Kits

500-fold expansion of Treg cells from CD4+ T cells in 9 days

RUO, GMP

Fully Humanized CD3 and CD28 Antibodies

ProDots Recombinant Human IL-2 GMP ProteinExCellerate Human T Cell Expansion Media, Recombinant Proteins, GMP Proteins

Cloudz Human NK Cell Expansion Kit

>100-fold expansion of NK cells in 10 days

RUO

Mouse CD2 and NKp46 Antibodies

Recombinant Human IL-2ExCellerate Human NK Cell Expansion Media, Recombinant Proteins

Cloudz Human NK Cell Expansion Kit (Coming Soon)

>100-fold expansion of NK cells in 10 days

RUO

Fully Humanized CD2 and NKp46 Antibodies

Recombinant Human IL-2ExCellerate Human NK Cell Expansion Media, Recombinant Proteins

Custom Cloudz Kits

If you already know which ligands you need to activate or expand your cells, our Custom Services team will work with you to produce a Cloudz kit based on those ligands. We have access to all Bio-Techne catalog products as well as thousands of non-catalog reagents. We can also custom design ligands such as xeno-free humanized antibodies. When you are ready to take the step to GMP-grade materials, we can deliver that too.

Inquire about Custom Cloudz
ScaleReady Logo

Bio-Techne believes in the power of partnerships. We have joined with Fresenius Kabi and Wilson Wolf to bring you ScaleReady for immune cell therapy manufacturing.

ScaleReady brings cell and gene therapies to life by streamlining manufacturing processes across cell culture, cell activation and expansion, gene editing, and cell processing.

Learn More at ScaleReady.com